rabbit polyclonal ab against survivin antibody  (Novus Biologicals)

Journal: Cancer Science

Article Title: Immunohistological analysis of pancreatic carcinoma after vaccination with survivin 2B peptide: Analysis of an autopsy series

doi: 10.1111/cas.14099

Figure Lengend Snippet: Immunohistochemistry for pancreatic carcinoma. A, HLA class I molecules in the surface of tumor cell. B, Survivin in the nucleus and/or cytoplasm of tumor cell. C, Programmed cell death‐1 ( PD ‐1) in the cell boundary of lymphocytes (arrows). D, Forkhead box P3 ( FOXP 3) in the nucleus of lymphocytes (arrows). E, PD ligand 1 ( PD ‐L1) in the surface of tumor cell. Bar = 50 μm. Original magnification, ×200

Article Snippet: Rabbit polyclonal Ab against survivin (2 μg/mL; Novus Biologicals, Littleton, CO, USA) was used.

Techniques: Immunohistochemistry

Journal: Cancer Science

Article Title: Immunohistological analysis of pancreatic carcinoma after vaccination with survivin 2B peptide: Analysis of an autopsy series

doi: 10.1111/cas.14099

Figure Lengend Snippet: Vaccination schedule for each patient included in the clinical trial who was subsequently investigated at autopsy and the distinctive infiltration pattern of CD 8 + cells in pancreatic carcinoma. A, In Step 1, the patients were treated until diagnosed as having progressive disease by RECIST or clinically apparent progressive disease. In Step 2, all patients who had given their consent were treated with survivin 2B peptide ( SVN ‐2B) and β‐interferon ( IFN β) until diagnosed as immune‐related response criteria (ir RC ) immune‐related progression of disease (ir PD ) or for a maximum 7 mo. B,C, Double staining pictures showing pan‐cytokeratin (red) expressed in tumor cells and CD 8 (brown) in lymphocytes. CD 8 + lymphocyte‐rich (B) and CD 8 + lymphocyte‐poor (C) tumor tissue specimens are shown. There is a small number of infiltrated CD 8 + lymphocytes in the CD 8 + lymphocyte‐poor lesion (C) identified as the internal positive control (arrows). Bar = 50 μm. Original magnification, ×200. D, Heterogenous distribution of CD 8 + lymphocytes in tumor tissue. Dotted line indicates the boundary between the tumor and interstitial tissue. Bar = 100 μm. Original magnification, ×100. E, Schematic diagram of lymphocyte infiltration into tumor tissue composed of tumor cells and interstitial tissue

Article Snippet: Rabbit polyclonal Ab against survivin (2 μg/mL; Novus Biologicals, Littleton, CO, USA) was used.

Techniques: Double Staining, Positive Control

Journal: Cancer Science

Article Title: Immunohistological analysis of pancreatic carcinoma after vaccination with survivin 2B peptide: Analysis of an autopsy series

doi: 10.1111/cas.14099

Figure Lengend Snippet: In situ investigation of infiltration of CD 8 + and programmed cell death‐1 ( PD ‐1) + lymphocytes into pancreatic carcinoma lesions. A,B, Comparison of infiltration of CD 8 + cells in the tumor (A) and interstitial tissue (B) between patients who had and had not received the survivin 2B peptide ( SVN ‐2B) vaccine. C, Number of CD 8 + lymphocytes that infiltrated each tumor lesion. D,E, Comparison of infiltration of PD ‐1 + cells into the tumor (D) and interstitial tissue (E) between patients who had and had not received the SVN ‐2B vaccine. F, Number of PD ‐1 + lymphocytes that infiltrated each lesion. G,H, Correlation between proportions of the PD ‐L1 + tumor and CD 8 + (G) or PD ‐1 + (H) lymphocyte infiltration in the lesions. PD ‐L1 expression on the surface of tumor cells was deemed to be positive. Signal intensity was not evaluated

Article Snippet: Rabbit polyclonal Ab against survivin (2 μg/mL; Novus Biologicals, Littleton, CO, USA) was used.

Techniques: In Situ, Comparison, Expressing

Journal: Cancer Science

Article Title: Immunohistological analysis of pancreatic carcinoma after vaccination with survivin 2B peptide: Analysis of an autopsy series

doi: 10.1111/cas.14099

Figure Lengend Snippet: Immunological effects in peripheral blood and histopathological analysis of pancreatic carcinoma tumor specimens from cases 2 and 7. A,E, Flow cytometry analysis of peripheral blood for CD 8 + T lymphocytes specific for survivin 2B peptide ( SVN ‐2B) peptide on the HLA ‐A24 complex before (left panel) and 8 weeks after (right panel) the first vaccination in case 2 (A) and case 7 (E). B,F, γ‐Interferon ( IFN γ) enzyme‐linked immunospot assay of PBMC s at 8 weeks after the first vaccination for no peptide (left), HIV (center), or SVN ‐2B (right) specific peptide in case 2 (B) and case 7 (F). C,G, Gross appearance of the cut surface of a liver lesion after formalin fixation in case 2 and before fixation in case 7 (G). Arrows indicate metastatic lesions. D,H, Morphological image (H&E stain; left panel) and immunohistochemistry for CD 8 (center panel) and programmed cell death ligand 1 ( PD ‐L1) (right panel) of a metastatic liver lesion in case 2 (D) and case 7 (H). Insets show high‐power magnification

Article Snippet: Rabbit polyclonal Ab against survivin (2 μg/mL; Novus Biologicals, Littleton, CO, USA) was used.

Techniques: Flow Cytometry, Enzyme-linked Immunospot, Staining, Immunohistochemistry